MOESM1 of Deletion of low-density lipoprotein-related receptor 5 inhibits liver Cancer cell proliferation via destabilizing Nucleoporin 37
journal contributionposted on 2019-12-28, 04:34 authored by Jinxiao Chen, Da Wo, En Ma, Hongwei Yan, Jun Peng, Weidong Zhu, Yong Fang, Dan-ni Ren
Additional file 1 : Figure S1. TOPflash assay showing the basal level of Wnt/β-catenin pathway activation between HCC cell line HepG2 cells and a non cancer cell line HEK293 cells. n = 3. Figure S2. Tumor formation assay following injection of HepG2 cells in SCID/bg mice after transient transfection with control, LRP5/6, or β-catenin siRNAs. n = 4. * p < 0.05, n.s. no significance, compared to si-Ctr. Figure S3. TOPflash assay showing the individual roles of LRP5 and LRP6 in regulating Wnt/β-catenin pathway. n = 3. Figure S4. Western blots showing the knockdown efficiency of all three LRP5 siRNAs, as well as LRP6 and β-catenin siRNAs. n = 3. GAPDH, loading control. Figure S5. Knockdown efficiency of all three NUP37 siRNAs as verified using real-time PCR assay (A), as well as western blot assay (B). n = 3. GAPDH, loading control. *** p < 0.001, compared to si-Ctr. Figure S6. MTT assay (A) and Photoimages (B) of Huh7 cell proliferation following knockdown of NUP37 or LRP5. n = 3. *** p < 0.001, compared to si-Ctr. Figure S7. Photoimages and quantification of HEK-293 cell proliferation following knockdown of NUP37. n = 3. ** p < 0.01, *** p < 0.001, compared to si-Ctr. Figure S8. MTT assay showing the decrease in cell proliferation following treatment with YAP inhibitor CA3(CIL56) in NUP37 knocked-down HepG2 cells. n = 3. ** p < 0.01, *** p < 0.001, compared to si-Ctr. Figure 9. Western blots showing the subcellular localization of LRP5 and NUP37. n = 3.