Laboratory protocol for the Agrobacterium-mediated transformation of Diplodia sapinea.
journal contribution
posted on 2024-12-27, 18:40 authored by Anne Geertje Oostlander, Laura Brodde, Miriam von Bargen, Bernard Slippers, Yvonne Becker, Ulrike Brandt, Frank Klawonn, Christiaan Grobler, Lucas Well, Jan Stenlid, Jonas Oliva, Malin Elfstrand, Andre FleissnerAccessible online at DOI: http://dx.doi.org/10.17504/protocols.io.5qpvok7ozl4o/v1.
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heterologous gene expressionfluorescent protein mcherrycell biology toolboxcell biology approachesconcept study enabledadditional experimental toolspinus sylvestris diplodia sapinea pinus sapinea planta agrobacterium tree deathsuccessful researchshoot blightsevere symptomspathogen systemmolecular geneticslikely dueincreasing threatincluding protocolshistone h2bharvested woodglobal ecosystemsfr .)forest healthfilamentous fungusefficient laboratorydespite extensivecurrently intensifiedclimate changeblue stain
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