oxley-investigatingboth-2020.pdf (2.77 MB)
Investigating both mucosal immunity and microbiota in response to gut enteritis in yellowtail kingfish
journal contribution
posted on 2020-01-01, 00:00 authored by T P R A Legrand, J W Wynne, L S Weyrich, Andrew OxleyAndrew OxleyThe mucosal surfaces of fish play numerous roles including, but not limited to, protection against pathogens, nutrient digestion and absorption, excretion of nitrogenous wastes and osmotic regulation. During infection or disease, these surfaces act as the first line of defense, where the mucosal immune system interacts closely with the associated microbiota to maintain homeostasis. This study evaluated microbial changes across the gut and skin mucosal surfaces in yellowtail kingfish displaying signs of gut inflammation, as well as explored the host gene expression in these tissues in order to improve our understanding of the underlying mechanisms that contribute to the emergence of these conditions. For this, we obtained and analyzed 16S rDNA and transcriptomic (RNA-Seq) sequence data from the gut and skin mucosa of fish exhibiting different health states (i.e., healthy fish and fish at the early and late stages of enteritis). Both the gut and skin microbiota were perturbed by the disease. More specifically, the gastrointestinal microbiota of diseased fish was dominated by an uncultured Mycoplasmataceae sp., and fish at the early stage of the disease showed a significant loss of diversity in the skin. Using transcriptomics, we found that only a few genes were significantly differentially expressed in the gut. In contrast, gene expression in the skin differed widely between health states, in particular in the fish at the late stage of the disease. These changes were associated with several metabolic pathways that were differentially expressed and reflected a weakened host. Altogether, this study highlights the sensitivity of the skin mucosal surface in response to gut inflammation.
History
Journal
MicroorganismsVolume
8Issue
9Season
Special Issue Host–Microbe Interactions in Animal/Human Health and DiseaseArticle number
1267Pagination
1 - 17Publisher
MDPILocation
Basel, SwitzerlandPublisher DOI
Link to full text
ISSN
2076-2607eISSN
2076-2607Language
engPublication classification
C Journal article; C1 Refereed article in a scholarly journalUsage metrics
Categories
No categories selectedKeywords
Licence
Exports
RefWorks
BibTeX
Ref. manager
Endnote
DataCite
NLM
DC