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Download fileInstant, Visual, and Instrument-Free Method for On-Site Screening of GTS 40-3‑2 Soybean Based on Body-Heat Triggered Recombinase Polymerase Amplification
journal contribution
posted on 27.03.2017, 00:00 authored by Rui Wang, Fang Zhang, Liu Wang, Wenjuan Qian, Cheng Qian, Jian Wu, Yibin YingOn-site
monitoring the plantation of genetically modified (GM)
crops is of critical importance in agriculture industry throughout
the world. In this paper, a simple, visual and instrument-free method
for instant on-site detection of GTS 40-3-2 soybean has been developed.
It is based on body-heat recombinase polymerase amplification (RPA)
and followed with naked-eye detection via fluorescent DNA dye. Combining
with extremely simplified sample preparation, the whole detection
process can be accomplished within 10 min and the fluorescent results
can be photographed by an accompanied smart phone. Results demonstrated
a 100% detection rate for screening of practical GTS 40-3-2 soybean
samples by 20 volunteers under different ambient temperatures. This
method is not only suitable for on-site detection of GM crops but
also demonstrates great potential to be applied in other fields.
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Keywords
20 volunteersinstrument-free method10 mindetection processRPADNA dyesample preparationnaked-eye detectionambient temperaturesGTS 40-3-2 soybean samplesOn-Site ScreeningInstrument-Free MethodBody-Heat Triggered Recombinase Polymerase Amplification On-site monitoringGTS 40-3-2 soybeanbody-heat recombinase polymerase amplificationagriculture industryGM crops