pone.0241576.s006.pdf (514.43 kB)
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Inhibition of the proteasome with MG-132 does not affect autophagy.

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journal contribution
posted on 23.12.2020, 19:34 authored by Nadine Camougrand, Pierre Vigié, Cécile Gonzalez, Stéphen Manon, Ingrid Bhatia-Kiššová

BY4742 (A) and atg32Δ mutant (B) cells expressing GFP-Atg8 protein grown in a CMS-L medium in presence or absence of MG-132 were harvested at indicated times. Total protein extracts from 2 x 107 cells were prepared and separated by 12.5% SDS-PAGE gel as described in the Material and Methods section. Proteins were detected using antibodies against GFP or Pgk1.