am8b04867_si_001.pdf (900.2 kB)
Download fileIn Vivo Imaging Tracking and Immune Responses to Nanovaccines Involving Combined Antigen Nanoparticles with a Programmed Delivery
journal contribution
posted on 2018-06-14, 00:00 authored by Xia Dong, Jie Liang, Afeng Yang, Chun Wang, Deling Kong, Feng LvCombined nanovaccine
can generate robust and persistent antigen-specific immune responses.
A combined nanovaccine was developed based on antigen-loaded genipin-cross-linked-polyethyleneimine–antigen
nanoparticles and in vivo multispectral fluorescence imaging tracked
the antigen delivery of combined nanovaccine. The inner layer antigen
nanoparticles carried abundant antigens by self-cross-linking for
persistent immune response, whereas the outer antigen on the surface
of antigen nanoparticles provided the initial antigen exposure. The
delivery of combined nanovaccine was tracked dynamically and objectively
by the separation of inner genipin cross-linked antigen nanoparticle
and the outer fluorescent antigen. The immune responses of the combined
nanovaccine were evaluated including antigen-specific CD4+ and CD8+ T-cell responses, IgG antibody level, immunological
memory, and CD8+ cytotoxic T lymphocyte responses. The
results indicated that the inner and outer antigens of combined vaccine
can be tracked in real time with a programmed delivery by the dual
fluorescence imaging. The programmed delivery of the inner and outer
antigens induced strong immune responses with a combination of a quick
delivery and a persistent delivery. With adequate antigen exposure,
the dendritic cells were effectively activated and matured, and following
T cells were further activated for immune response. Compared with
a single nanoparticle formulation, the combined nanovaccine exactly
elicited a stronger antigen-specific immune response.