Joss_et_al_1996.pdf (1.84 MB)
In Vitro Biosynthesis of Androgens in the Australian Lungfish, Neoceratodus forsteri
journal contributionposted on 2023-05-16, 17:50 authored by Joss, JMP, Ashley EdwardsAshley Edwards, Kime, DE
The serum concentration of testosterone was estimated from a population of wild lungfish over 6-7 years of sampling. Male lungfish were found to have high circulating levels of testosterone (~50 ng/ml) which varied seasonally and could be correlated with spermatogenesis as judged by testis histology. Incubation of testis tissue slices with [ 3H]progesterone, [ 3H]17- hydroxyprogesterone, or [ 3H]testosterone confirmed that testosterone is the major androgen in Neoceratodus. Not even trace amounts of 11-keto- or 11Î²- hydroxytestosterone or 5Î±-dihydrotestosterone could be identified by TLC separations. There was little or no conjugation of steroids by the testes, except during the spawning season, when glucuronides of androstenedione and testosterone were produced.
Publication titleGeneral and Compartive Endocrinology
Department/SchoolSchool of Natural Sciences
PublisherAcademic Press Inc
Place of publicationUSA