bm2c01277_si_001.pdf (639.29 kB)
Glycosaminoglycan Mimetics Obtained by Microwave-Assisted Sulfation of Marine Bacterium Sourced Infernan Exopolysaccharide
journal contributionposted on 2022-12-23, 22:29 authored by Sylvia Colliec-Jouault, Fabiana Esposito, Hélène Ledru, Corinne Sinquin, Laetitia Marchand, Arnaud Fillaudeau, Sylvain Routier, Frédéric Buron, Chrystel Lopin-Bon, Stéphane Cuenot, Emiliano Bedini, Agata Zykwinska
Sulfated glycosaminoglycans (GAGs) are fundamental constituents of both the cell surface and extracellular matrix. By playing a key role in cell–cell and cell–matrix interactions, GAGs are involved in many physiological and pathological processes. To design GAG mimetics with similar therapeutic potential as the natural ones, the specific structural features, among them sulfate content, sulfation pattern, and chain length, should be considered. In the present study, we describe a sulfation method based on microwave radiation to obtain highly sulfated derivatives as GAG mimetics. The starting low-molecular-weight (LMW) derivative was prepared from the infernan exopolysaccharide, a highly branched naturally slightly sulfated heteropolysaccharide synthesized by the deep-sea hydrothermal vent bacterium Alteromonas infernus. LMW highly sulfated infernan derivatives obtained by conventional heating sulfation have already been shown to display GAG-mimetic properties. Here, the potential of microwave-assisted sulfation versus that of the conventional method to obtain GAG mimetics was explored. Structural analysis by NMR revealed that highly sulfated derivatives from the two methods shared similar structural features, emphasizing that microwave-assisted sulfation with a 12-fold shorter reaction time is as efficient as the classical one.
highly sulfated derivativesglycosaminoglycan mimetics obtainedspecific structural featurescell – cellobtain gag mimeticsdesign gag mimeticssulfation method basedconventional heating sulfationassisted sulfation versussimilar therapeutic potentialgag mimeticsassisted sulfationconventional methodcell surfacesulfation patternstructural analysisdisplay gagsulfate contentstarting lowpresent studypathological processesnmr revealednatural onesmimetic propertiesmany physiologicalkey roleinfernan exopolysaccharidefundamental constituentsextracellular matrixclassical onechain length