figshare
Browse

File(s) not publicly available

Genome-wide DNA methylation analysis: no evidence for stable hemimethylation in the sheep muscle genome

journal contribution
posted on 2023-05-03, 22:10 authored by Christine Couldrey, Rudiger BrauningRudiger Brauning, Harold HendersonHarold Henderson, John McEwanJohn McEwan
The importance of maintaining DNA methylation patterns and faithful transmission of these patterns during cell division to ensure appropriate gene expression has been known for many decades now. It has largely been assumed that the symmetrical nature of CpG motifs, the most common site for DNA methylation in mammals, together with the presence of maintenance methylases able to methylate newly synthesised DNA, ensures that there is concordance of methylation on both strands. However, although this assumption is compelling in theory, little experimental evidence exists that either supports or refutes this assumption. Here, we have undertaken a genome-wide single-nucleotide resolution analysis to determine the frequency with which hemimethylated CpG sites exist in sheep muscle tissue. Analysis of multiple independent samples provides strong evidence that stably maintained hemimethylation is a very rare occurrence, at least in this tissue. Given the rarity of stably maintained hemimethylation, next-generation sequencing data from both DNA strands may be carefully combined to increase the accuracy with which DNA methylation can be measured at single-nucleotide resolution.

History

Rights statement

© 2015 Stichting International Foundation for Animal Genetics

Language

  • English

Does this contain Māori information or data?

  • No

Publisher

Wiley

Journal title

Animal Genetics

Citation

Couldrey, C., Brauning, R., Henderson, H. V., & McEwan, J. C. (2015). Genome-wide DNA methylation analysis: no evidence for stable hemimethylation in the sheep muscle genome. Animal Genetics, 46(2), 185-189.

Usage metrics

    Exports

    RefWorks
    BibTeX
    Ref. manager
    Endnote
    DataCite
    NLM
    DC