Figure S1. Lithocolla globosa

Figure S1. Lithocolla globosa SnP. A-B) Agglutinated cells from enrichment with sediment still
present. Surface view (B) showing attached mineral particles. C-O) Cells from culture, fed on
Navicula (C-G), Isochrysis (H,L-O) and Phaeodactylum (I-K). C-D) General view of same cell.
Note nucleus (in C) and ingested diatoms (in D), orange globules (D inset, bracket) and layer
(D inset, arrowhead). E) General view of cell. Note nucleus, orange globules and greenish
digestive vacuoles. F-H) Examples of intact filopodia. Note that filopodia degrade rapidly under
exposure to bright light. I-J) Individuals with 2 (I) and 3 (J) nuclei, relatively commonly observed
in culture. K) 'Re-agglutinated' cell one day following addition of chalk dust (as fine calcium
carbonate mineral inclusions) to culture. Note mineral particles inside and on the surface of the
cell, resembling the agglutinated cells from enrichment in (A-B). L-O) Pseudoseries of different
individuals showing mineral inclusions found in cells (arrowheads), 20min (L), 23min (M), 47min
(N), 60min (O) following addition of chalk dust to mineral-particle-deprived culture, indicating that
mineral particles are ingested by the cell. Differential interference contrast, scalebars are 20μm
(A-K, all images at same scale), 10μm (L-O).


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