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Feasibility study on the use of attenuated total reflectance infrared spectroscopy as high throughput screening tool to phenotype single barley seeds (Hordeum vulgare L.)

journal contribution
posted on 19.09.2018, 00:00 authored by Daniel CozzolinoDaniel Cozzolino, S Roumeliotis, J Eglinton
Knowledge of the chemical and structural differences between barley (Hordeum vulgare L.) varieties and breeding lines might lead to an understanding of the reasons for these differences among genotypes in relation to their malting properties. The aim of this study was to evaluate the use of attenuated total reflectance mid infrared (ATR-MIR) spectroscopy combined with univariate and multivariate methods as a high throughput screening tool to phenotype single seeds of barley. Varieties that yield high malting quality (hot water extract (HWE) > 80%) showed relatively high non-structural carbohydrates (e.g. starch) to protein and non-structural to structural (e.g. cellulose) carbohydrates ratios compared with those samples having moderate HWE (78% < HWE < 80%). The use of ATR-MIR spectroscopy and in particular the use of ratios at specific wavenumbers can provide useful information about the biophysical and chemical characteristics of the grain.

Funding

Category 2 - Other Public Sector Grants Category

History

Volume

116

Issue

4

Start Page

379

End Page

384

Number of Pages

6

ISSN

1537-5110

Publisher

Elsevier

Peer Reviewed

Yes

Open Access

No

Acceptance Date

11/08/2013

External Author Affiliations

The University of Adelaide

Era Eligible

Yes

Journal

Biosystems Engineering