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journal contribution
posted on 11.03.2016, 01:04 by Andrés González, M. Teresa Bes, M. Luisa Peleato, María F. Fillat

Figure A: Expression of FurA results essential to the growth of Anabaena sp. under standard culture conditions. Figure B: Fold changes in the expression of FurA direct targets in the furA-turning off strain Anabaena sp. AGcoaRFurA after cobalt/zinc deprivation as compared with the wild-type PCC 7120 strain under the same growth condition, as result of semi-quantitative RT-PCR analyses. Figure C: Fold changes in the expression of FurA direct targets in the furA-overexpressing strain Anabaena sp. AG2770FurA under iron-replete condition (BG-11 medium) as compared with the wild-type PCC 7120 strain under the same growth condition, as result of semi-quantitative RT-PCR analyses. Figure D: Fold changes in the expression of FurA direct targets in the wild-type strain Anabaena sp. PCC 7120 after iron deprivation as compared with the same strain under iron-replete condition (BG-11 medium), as result of semi-quantitative RT-PCR analyses. Figure E: Fold changes in the expression of FurA direct targets in the furA-overexpressing strain Anabaena sp. AG2770FurA under iron-replete condition (BG-11 medium) as compared with the wild-type PCC 7120 strain grown under iron deprivation, as result of semi-quantitative RT-PCR analyses. Figure F: Construction of Anabaena sp. strain AGcoaRFurA. Table A: Protein sequences producing significant alignments (≥80% identity) with FurA from Anabaena sp. PCC 7120. Table B: Complete list of genes showing ≥2-fold change in expression in the furA-turning off strain AGcoaRFurA. Table C: Genes showing <2-fold change in expression in the furA-turning off strain AGcoaRFurA. Table D: Main genes involved in iron uptake showing ≥2-fold change in expression in the furA-turning off strain AGcoaRFurA. Table E: Strains and plasmids used in this study. Table F: Oligonucleotides used in this study. Table G: Summary of sequencing run statistics

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