Evolution of the Cannabinoid and Terpene Content (JNP 2016).pdf (3.87 MB)

Evolution of the Cannabinoid and Terpene Content during the Growth of Cannabis sativa Plants from Different Chemotypes

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journal contribution
posted on 2017-05-22, 12:46 authored by Oier Aizpurua-OlaizolaOier Aizpurua-Olaizola, U. Soydaner, E. Öztürk, D. Schibano, Y. Simsir, P. Navarro, N. Etxebarria, A. Usobiaga
The evolution of major cannabinoids and terpenes during
the growth of Cannabis sativa plants was studied. In this work, seven
different plants were selected: three each from chemotypes I and III and
one from chemotype II. Fifty clones of each mother plant were grown
indoors under controlled conditions. Every week, three plants from each
variety were cut and dried, and the leaves and flowers were analyzed
separately. Eight major cannabinoids were analyzed via HPLC-DAD, and
28 terpenes were quantified using GC-FID and verified via GC-MS. The
chemotypes of the plants, as defined by the tetrahydrocannabinolic acid/
cannabidiolic acid (THCA/CBDA) ratio, were clear from the beginning
and stable during growth. The concentrations of the major cannabinoids
and terpenes were determined, and different patterns were found among
the chemotypes. In particular, the plants from chemotypes II and III
needed more time to reach peak production of THCA, CBDA, and monoterpenes. Differences in the cannabigerolic acid
development among the different chemotypes and between monoterpene and sesquiterpene evolution patterns were also
observed. Plants of different chemotypes were clearly differentiated by their terpene content, and characteristic terpenes of each
chemotype were identified.