temi_a_1830715_sm0876.docx (256.75 kB)

Establishment of replication-competent vesicular stomatitis virus-based recombinant viruses suitable for SARS-CoV-2 entry and neutralization assays

Download (256.75 kB)
journal contribution
posted on 17.10.2020 by Hongyue Li, Chaoyue Zhao, Yuhang Zhang, Fei Yuan, Qi Zhang, Xuanling Shi, Linqi Zhang, Chengfeng Qin, Aihua Zheng

Replication-competent vesicular stomatitis virus (VSV)-based recombinant viruses are useful tools for studying emerging and highly pathogenic enveloped viruses in level 2 biosafety facilities. Here, we used a replication-competent recombinant VSVs (rVSVs) encoding the spike (S) protein of SARS-CoV-2 in place of the original G glycoprotein (rVSV-eGFP-SARS-CoV-2) to develop a high-throughput entry assay for SARS-CoV-2. The S protein was incorporated into the recovered rVSV-eGFP-SARS-CoV-2 particles, which could be neutralized by sera from convalescent COVID-19 patients. The recombinant SARS-CoV-2 also displayed entry characteristics similar to the wild type virus, such as cell tropism and pH-dependence. The neutralizing titers of antibodies and sera measured by rVSV-eGFP-SARS-CoV-2 were highly correlated with those measured by wild-type viruses or pseudoviruses. Therefore, this is a safe and convenient screening tool for SARS-CoV-2, and it may promote the development of COVID-19 vaccines and therapeutics.

Funding

This project was supported by the National Key Plan for Scientific Research and Development of China (Grant no. 2016YFD0500303); the National Science and Technology Major Project (Grant no. 2018ZX10101004); and National Natural Science Foundation of China, General Program (Grant no. 81871687).

History

Licence

Exports