Engineering Camel Single-Domain Antibodies and Immobilization Chemistry for Human Prostate-Specific Antigen Sensing
journal contributionposted on 01.12.2005, 00:00 by Dirk Saerens, Filip Frederix, Gunter Reekmans, Katja Conrath, Karolien Jans, Lea Brys, Lieven Huang, Eugène Bosmans, Guido Maes, Gustaaf Borghs, Serge Muyldermans
The specificity and affinity characteristics of antibodies make them excellent probes in biosensor applications. Unfortunately, their large size, unstable behavior, and random immobilization properties create numerous problems. The single-domain antigen-binding fragment derived from heavy-chain antibodies of camelids (termed VHH) offers special advantages in terms of size, stability, and ease of generating different antibody constructs. In this study, we show the potential of those VHHs in sensing human prostate-specific antigen (hPSA) by SPR technology. Different VHH constructs were immobilized onto commercial and custom-built sensor surfaces by metal chelation, biotin−streptavidin interaction, or covalent coupling. The detection of subnanogram per milliliter hPSA concentrations could be attained on a covalently coupled three-dimensional dextran surface. Moreover, the ratio of different hPSA isoform concentrations could be assessed via a sandwich assay and resulted in the detection of clinically significant antigen concentrations within 15 min. In addition, for the first time, the intrinsic protein stability is presented as an important probe design factor, since our results reveal that higher intrinsic stability offers higher resistance to harsh regeneration conditions. In conclusion, we present VHHs as a novel class of biosensor probes rivaling conventional antibodies and their derived antibody fragments.
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probe design factorantibody fragmentsdextran surface15 minantigen concentrationsbiosensor probesdetectionhPSA isoform concentrationsprotein stabilitySPR technologyImmobilization ChemistryDifferent VHHaffinity characteristicssensor surfacesmetal chelationregeneration conditionsmilliliter hPSA concentrationsnovel classbiosensor applicationsimmobilization propertiessandwich assay