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ENaC and CFTR expression and effects of amiloride and amlodipine on blood pressure and sweat excretion.

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posted on 2021-02-16, 18:48 authored by Meiling He, Tiantian Zhou, Yayan Niu, Wansheng Feng, Xiabing Gu, Wenting Xu, Shengnan Zhang, Zhiting Wang, Yue Zhang, Can Wang, Liang Dong, Meng Liu, Ningzheng Dong, Qingyu Wu

(A) Quantitative RT-PCR analysis of Scnn1b mRNA, encoding β-ENaC, in footpads from WT and corin KO mice on 0.3% salt diet. (B) Western blotting of β-ENaC protein in footpads from WT and corin KO mice (n = 4 per group) on 0.3 salt diet. Proteins expression levels were quantified by densitometric analysis of the western blot. (C) Systolic BP in WT and corin KO mice on 0.3% or 4% salt diet treated with vehicle or amiloride. (D) Systolic BP in WT and corin KO mice on 0.3% salt diet treated with vehicle or amlodipine. (E) Sweat excretion was analyzed by the iodine–starch test in WT and corin KO mice on 0.3% salt diet and treated with (+) or without (−) amlodipine and pilocarpine (pilocar). (F) Quantitative RT-PCR analysis of Cftr mRNA, encoding CFTR, in footpads from WT and corin KO mice on 0.3% salt diet. All data are mean ± SEM. P values were analyzed by 2-tailed Student t test (in A, C, and E) or 1-way ANOVA (in B and D). In bar graphs, each dot represents data from 1 mouse, and the original numerical values are in S1 Data. BP, blood pressure; CFTR, cystic fibrosis transmembrane conductance regulator; ENaC, epithelial sodium channel; KO, knockout; RT-PCR, reverse transcription PCR; WT, wild-type.

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