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Development and Characterizations of a Miniature Capillary Electrophoresis Mass Spectrometry System

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journal contribution
posted on 17.02.2015, 00:00 by Muyi He, Zhenhua Xue, Yinna Zhang, Zejian Huang, Xiang Fang, Feng Qu, Zheng Ouyang, Wei Xu
A miniature capillary electrophoresis mass spectrometry (CE/MS) system has been developed in this work. A 100% electrical driven miniaturized CE device was integrated with a miniature MS instrument, which has a discontinuous atmospheric pressure interface (DAPI) for coupling with atmospheric pressure ionization sources. A nanoelectrospray ionization (nano-ESI) source was developed with a sheath liquid interface for coupling the miniature CE and the MS system. A systematic characterization and optimization of the analytical performance have been done. The analysis of isobaric peptides and avoiding charge competition effects in nano-ESI sources have been demonstrated.