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Detection and Identification of Bioanalytes with High Resolution LSPR Spectroscopy and MALDI Mass Spectrometry

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journal contribution
posted on 2009-04-16, 00:00 authored by Jeffrey N. Anker, W. Paige Hall, Mary P. Lambert, Pauline T. Velasco, Milan Mrksich, William L. Klein, Richard P. Van Duyne
High resolution localized surface plasmon resonance (HR-LSPR) sensors were combined with matrix assisted laser desorption ionization mass spectrometry (MALDI-MS) for the first time. LSPR sensors provide real-time label-free detection of molecular adsorption. Subsequent MALDI-MS analysis enables identification of the adsorbed molecules. This synergistic LSPR-MS approach was applied to the detection and identification of amyloid beta oligomers which play an important role in the molecular pathogenesis of Alzheimer’s Disease.