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Cellular Metabolism of Fluorescent Nanoprobes Formed by Self-Assembly of Amphiphiles: Dynamic Trafficking from the Golgi Apparatus to the Lysosome

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journal contribution
posted on 14.11.2019, 17:36 by Shixin Zhou, Changwen Deng, Pan Xu, Qi Fan, Xiaoyan Zhang, Yongrui Jia, Li Su, Qihua He, Yinan Liu, Bo Song
Dynamic trafficking of foreign substances (organic molecules) in eukaryotic cells is closely correlated to the metabolism of cells; however, it is to date not fully understood due to the lack of suitable probes. In the present study, we employed an amphiphilic probe (i.e., TPE-11) with aggregation induced emission properties to study the trafficking, and through cutting the feed of TPE-11 after a certain culturing time, we confirmed that the trafficking and aggregation of the amphiphilic dye molecules are not controlled by the entropy-driven diffusion but mainly determined by the biological activities of the cells. In addition, the trafficking of the nanoprobes formed by TPE-11 was analyzed by colocalizing the fluorescent signals of GM130/calnexin (anchoring proteins of the Golgi apparatus (GOL) and endoplasmic reticulum (ER), respectively) and TPE-11. In the first 5 h (after removal of the dyes), the fluorescent signals of the nanoemitters were mainly localized at GOL rather than ER, and in the second 6 h, the signals migrated to the lysosome. Moreover, suppression of the protein transporting function led to a random distribution of nanoprobes all over the cells. We assume that GOL should be a main organelle for aggregation of TPE-11. Thereafter, the aggregates were then transferred to the lysosome for further processing.

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