es8b01150_si_001.pdf (1.46 MB)
Download fileAlterations to the Intestinal Microbiome and Metabolome of Pimephales promelas and Mus musculus Following Exposure to Dietary Methylmercury
journal contribution
posted on 2022-01-25, 18:36 authored by Kristin M. Nielsen, Yan Zhang, Thomas E. Curran, Jason T. Magnuson, Barney J. Venables, Katherine E. Durrer, Michael S. Allen, Aaron P. RobertsMercury is a global contaminant, which may be microbially transformed
into methylmercury (MeHg), which bioaccumulates. This results in potentially
toxic body burdens in high trophic level organisms in aquatic ecosystems
and maternal transfer to offspring. We previously demonstrated effects
on developing fish including hyperactivity, altered time-to-hatch,
reduced survival, and dysregulation of the dopaminergic system. A
link between gut microbiota and central nervous system function in
teleosts has been established with implications for behavior. We sequenced
gut microbiomes of fathead minnows exposed to dietary MeHg to determine
microbiome effects. Dietary exposures were repeated with adult CD-1
mice. Metabolomics was used to screen for metabolome changes in mouse
brain and larval fish, and results indicate effects on lipid metabolism
and neurotransmission, supported by microbiome data. Findings suggest
environmentally relevant exposure scenarios may cause xenobiotic-mediated
dysbiosis of the gut microbiome, contributing to neurotoxicity. Furthermore,
small-bodied teleosts may be a useful model species for studying certain
types of neurodegenerative diseases, in lieu of higher vertebrates.
History
Usage metrics
Read the peer-reviewed publication
Categories
Keywords
useful model speciesstudying certain typespreviously demonstrated effectspimephales promelas </mus musculus </fathead minnows exposedsequenced gut microbiomesdetermine microbiome effectsresults indicate effectsdietary methylmercury mercurybodied teleosts maygut microbiomegut microbiotadietary mehgdietary exposuresmicrobiome dataintestinal microbiomereduced survivalneurodegenerative diseasesmouse brainmicrobially transformedmehg ),mediated dysbiosismaternal transferlipid metabolismlarval fishhigher vertebratesglobal contaminantfollowing exposuredopaminergic systemaquatic ecosystemsaltered timeadult cd1 mice