Additional file 7 of Real-time tracking of stem cell viability, proliferation, and differentiation with autonomous bioluminescence imaging

Additional file 7: Fig. S7. Immunohistochemical confirmation of cardiac differentiation. PDF File showing staining of cardiomyocytes with the anti-Troponin-T antibody to confirm successful differentiation. Following the onset of beating, cardiomyocyte differentiation was confirmed by staining with the primary antibody: Troponin T, Cardiac Isoform Ab-1, Mouse Monoclonal Antibody, Clone: 13-11 Isotype: IgG1 and visualizing with the secondary antibody: Goat anti Mouse IgG (H+L) Alexa Fluor 488. Cells were imaged using both the transmitted light and green fluorescent protein (GFP) channels of an EVOS M5000 Cell Imaging System at 40× magnification.