13024_2017_216_MOESM4_ESM.pdf (1.93 MB)

Additional file 4: of TREM2 deficiency exacerbates tau pathology through dysregulated kinase signaling in a mouse model of tauopathy

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journal contribution
posted on 2017-10-16, 05:00 authored by Shane Bemiller, Tyler McCray, Kevin Allan, Shane Formica, Guixiang Xu, Gina Wilson, Olga Kokiko-Cochran, Samuel Crish, Cristian Lasagna-Reeves, Richard Ransohoff, Gary Landreth, Bruce Lamb
A,B Western blot analysis reveals no alterations in cortical or hippocampal signaling molecules between 6-month non-transgenic BL6 control mice and Trem2 −/− mice. Additionally, no significant alterations in p-tau (C) were detected between BL6 and Trem2 −/− genotypes. Modest non-significant increases in total tau (Tau5) were detected in Trem2 −/− mice compared to BL6 non-transgenic mice. (D) Morphological analysis of Iba1 staining demonstrates no alterations among microglia between BL6 and Trem2 −/− control mice (N = 4 mice per genotype). At least two independent experiments were performed for each analysis. Error bars represent SEM. *, P < 0.05, **, P < 0.01, ***, P < 0.001. (PDF 1980 kb)