Additional file 3 of Development of a CRISPR/Cpf1 system for targeted gene disruption in Aspergillus aculeatus TBRC 277

Additional file 3: Fig. S3. The functional plasmid construction of CRISPR/Cpf1 system. The plasmid consists of Cpf1 nuclease gene and crRNA-pyrG cassettes for targeted genome modification in A. aculeatus TBRC 277. (a) pCRISPR/Cpf1-pyrG plasmid, crRNA is expressed under control of U3-AF pol. III promoter, (b) Guide RNA cassette, crRNA, consists of 19-bp direct repeats (DR) to form a stem-loop structure, as well as 20-bp pyrG-targeted protospacers.