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Additional file 2 of Unique Glutelin Expression Patterns and Seed Endosperm Structure Facilitate Glutelin Accumulation in Polyploid Rice Seed

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posted on 2021-07-06, 03:33 authored by Lu Gan, Baosheng Huang, Zhaojian Song, Yachun Zhang, Yujie Zhang, Si Chen, Liqi Tong, Zhisong Wei, Lingxiang Yu, Xiangbo Luo, Xianhua Zhang, Detian Cai, Yuchi He
Additional file 2: Table S1. Classification of rice glutelin genes. Table S2. Total protein content and average growth of tetraploid brown rice seeds and the corresponding diploid rice seeds harvested in Wuhan between November 2018 and November 2019. Data are presented as means ± standard errors of three biological replicates; * denotes significant differences in protein content between tetraploid and diploid rice (p < 0.05); ** denotes extremely significant differences (p < 0.01). Table S3. Glutelin and prolamin contents in tetraploid and diploid rice seeds (harvested in 2019). * denotes significant differences in component protein content between tetraploid and diploid rice (p < 0.05); ** denotes extremely significant differences (p < 0.01). Table S4. Seventeen amino acid contents of tetraploid and the corresponding diploid brown rice seeds (harvested in 2019). Data are presented means ± standard errors of three biological replicates; * denotes significant differences in amino acid contents between tetraploid and the corresponding diploid rice (p < 0.05); ** denotes extremely significant differences (p < 0.01). Table S5. Amylose contents of tetraploid and diploid rice (harvested in 2019). Data are presented as means ± standard errors of three biological replicates. * denotes significant differences in amylose contents between tetraploid and diploid rice (p < 0.05). ** denotes extremely significant differences (p < 0.01). Table S6. Synthetic peptides for polyclonal anti-glutelin antibodies. Table S7. Primer sequences used for qRT-PCR.

Funding

Chinese National Natural Science Foundation 2017 Hubei Science and Technology Department Innovation Team 2016 Wuhan Yellow Crane Talents (science) Foundation the Open Research Fund of State Key Laboratory of Hybrid Rice (Hunan Hybrid Rice Research Center) Wuhan Science and Technology Major Project “Development and application of polyploid rice (2016-2020)”

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