12896_2019_520_MOESM2_ESM.pdf (478.11 kB)
Download file

Additional file 2: of Surface patches on recombinant erythropoietin predict protein solubility: engineering proteins to minimise aggregation

Download (478.11 kB)
journal contribution
posted on 09.05.2019, 05:00 by M. Carballo-Amador, Edward McKenzie, Alan Dickson, Jim Warwicker
Table S1. Positively-charged patches size profile of rHuEPO WT from the charged patch calculator. Complete screening of posQ ratio scores for the modified rHuEPO WT (PDB: 1EER) is shown. The largest positive patches are represented by blue (ratio > 1.0). Those proteins with ratio above 1.0 are predicted as insoluble and below 1.0 as soluble. The three targeted residues in this study are highlighted in red. Ratio: largest positively-charged patch (posQ) value from the charged patch calculator [29]. Charge patches: HYD, hydrophobic (non-charged); NEG, negatively-charged; POS, positively-charged. (PDF 478 kb)