Additional file 2: of Quantitative evaluation of protocorm growth and fungal colonization in Bletilla striata (Orchidaceae) reveals less-productive symbiosis with a non-native symbiotic fungus

Sample preparation for quantitative evaluation of symbiotic cells in a protocorm. (a) A stained protocorm with ink solution. (b) A protocorm with testa removed. (c) Application of pressure to disassemble protocorm cells into the single layer. (d) Disassembled cells of a protocorm. Scale bars, 500 Οm. (PDF 530 kb)