12870_2017_1002_MOESM2_ESM.pdf (530.61 kB)

Additional file 2: of Quantitative evaluation of protocorm growth and fungal colonization in Bletilla striata (Orchidaceae) reveals less-productive symbiosis with a non-native symbiotic fungus

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journal contribution
posted on 2017-02-21, 05:00 authored by Tatsuki Yamamoto, Chihiro Miura, Masako Fuji, Shotaro Nagata, Yuria Otani, Takahiro Yagame, Masahide Yamato, Hironori Kaminaka
Sample preparation for quantitative evaluation of symbiotic cells in a protocorm. (a) A stained protocorm with ink solution. (b) A protocorm with testa removed. (c) Application of pressure to disassemble protocorm cells into the single layer. (d) Disassembled cells of a protocorm. Scale bars, 500 Οm. (PDF 530 kb)


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