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Additional file 2 of Development of a CRISPR/Cpf1 system for targeted gene disruption in Aspergillus aculeatus TBRC 277

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posted on 12.02.2021, 04:34 by Dede Abdulrachman, Lily Eurwilaichitr, Verawat Champreda, Duriya Chantasingh, Kusol Pootanakit
Additional file 2: Fig. S2. Western blot detection of heterologous EGFP and Cpf1-EGFP proteins in recombinant A. aculeatus TBRC 277 egfp and Cpf1-egfp, respectively. (a) GenCRISPRTM FnCpf1 monoclonal antibody (9H6) was used as the primary detection, 1.0 μg (Genscript, USA). (b) GFP monoclonal antibody (C163) was used for the primary detection, 3 μg (Thermo Fisher, USA). All samples were treated by IgG-AP as the secondary antibody for detection, 2 μg. Lane 1, crude protein of A. aculeatus recombinant (Cpf1-egfp). Lane 2, crude protein of recombinant A. aculeatus (egfp), Lane 3, crude protein of A. aculeatus TBRC 277 wild-type (control). Each lane was loaded with 20-μg protein. Anti-GFP (Roche) and FnCpf1 (Genscript, USA) antibodies were used as the primary (monoclonal) antibodies, anti IgG-conjugated AP was used for secondary antibody. M: PageRule Plus Prestained Protein Ladder (Thermo Fisher, USA).

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