12896_2020_608_MOESM2_ESM.pdf (145.78 kB)
Download file

Additional file 2 of An efficient gene disruption method for the woody plant pathogen Botryosphaeria dothidea

Download (145.78 kB)
journal contribution
posted on 06.03.2020, 04:37 authored by Bao-Zhu Dong, Li-Yun Guo
Additional file 2: Fig. S2. Verification of the Bdo_05381 GD B. dothidea ZY7 transformants. Five Bdo_05381-disrupted transformants were analyzed with four PCR amplifications and a Southern blot. a All transformants comprised the correct upstream (left) and downstream (right) fragments. b Absence of the ORF fragment in the transformants. c Longer whole-length fragment in the transformants than in the WT control. d Southern blot analysis of the hph insertion loci. Lanes 1–5 in a–d correspond to five GD transformants. Lane 6 corresponds to the linearized plasmid (11 kbp).

Funding

National Key R&D Program of China

History