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Additional file 2 of An efficient gene disruption method for the woody plant pathogen Botryosphaeria dothidea

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posted on 06.03.2020, 04:37 authored by Bao-Zhu Dong, Li-Yun Guo
Additional file 2: Fig. S2. Verification of the Bdo_05381 GD B. dothidea ZY7 transformants. Five Bdo_05381-disrupted transformants were analyzed with four PCR amplifications and a Southern blot. a All transformants comprised the correct upstream (left) and downstream (right) fragments. b Absence of the ORF fragment in the transformants. c Longer whole-length fragment in the transformants than in the WT control. d Southern blot analysis of the hph insertion loci. Lanes 1–5 in a–d correspond to five GD transformants. Lane 6 corresponds to the linearized plasmid (11 kbp).


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