12985_2016_488_MOESM2_ESM.pdf (253.3 kB)
Additional file 2: Figure S2. of Molecular epidemiology and evolutionary histories of human coronavirus OC43 and HKU1 among patients with upper respiratory tract infections in Kuala Lumpur, Malaysia
journal contribution
posted on 2016-02-25, 05:00 authored by Maryam Al-Khannaq, Kim Ng, Xiang Oong, Yong Pang, Yutaka Takebe, Jack Chook, Nik Hanafi, Adeeba Kamarulzaman, Kok TeePhylogenetic analysis of the HCoV-OC43 nucleocapsid gene. Trees were reconstructed using neighbor-joining method. Bootstrap values were calculated from 1,000 trees. Bootstrap values of greater than 70 % were indicated on the branch nodes. The scale bar of individual tree was indicated in substitutions per site, using Kimura 2-parameter model in MEGA (version 5.1) to estimate pair-wise evolutionary distance. The Malaysian isolates obtained in this study were color-coded and the HCoV-OC43 genotypes A to E as well as novel lineages 1 and 2 were indicated. Each HCoV-OC43 sequence was assigned to its proper genotype based on the S1 phylogenetic analysis. NL1= novel lineage 1. (PDF 253 kb)