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Additional file 1: of The genome of the biting midge Culicoides sonorensis and gene expression analyses of vector competence for bluetongue virus

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posted on 22.08.2018, 05:00 by Ramiro Morales-Hojas, Malcolm Hinsley, Irina Armean, Rhiannon Silk, Lara Harrup, Asier Gonzalez-Uriarte, Eva Veronesi, Lahcen Campbell, Dana Nayduch, Christopher Saski, Walter Tabachnick, Paul Kersey, Simon Carpenter, Mark Fife
Supplementary Methods. DNA extraction. Protocol followed to extract DNA from C. sonorensis samples. Genome annotation and comparative analysis. Details about the genomes of species used in the gene annotation and in the comparative analyses. RNA extraction. Protocol used to extract RNA from C. sonorensis. RT-qPCR for the detection of BTV-1 infection. Description of the method used to detect the presence of BTV-1 in C. sonorensis females after the experimental infections. RT-qPCR protocol for the validation of differentially expressed genes. Description of the protocol used to verify the change in expression of Toll-like protein, glutathione-s-transferase, glutathione-s-transferase 1 and ski2 antiviral helicase genes observed in the transcriptomes of vector-competent and refractory samples. Alignment of gst-1 and ski2. The commands used to align the protein sequences of gst-1 and ski2. These alignments were then used to run the phylogenetic analyses. (ZIP 117 kb)


Biotechnology and Biological Sciences Research Council