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Additional file 1 of Poly (N-vinylpyrrolidone) modification mitigates plasma protein corona formation on phosphomolybdate-based nanoparticles

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journal contribution
posted on 24.12.2021, 04:15 by Youyi Yu, Behafarid Ghalandari, Guangxia Shen, Liping Wang, Xiao Liu, Aiting Wang, Sijie Li, Haiyang Xie, Xianting Ding
Additional file 1: Figure S1 TEM images of the CDS-PMo12@PVP0 NPs. Figure S2 Size distributions of CDS-PMo12@PVPx (x=0~1) NPs. (a) CDS-PMo12@PVP0, (b) CDS-PMo12@PVP0.05, (c) CDS-PMo12@PVP0.1, (d) CDS-PMo12@PVP0.25, (e) CDS-PMo12@PVP0.5 and (f) CDS-PMo12@PVP1. Figure S3 FTIR spectra of the PVP, H3PMo12O30, CDS-PMo12@PVP0, CDS-PMo12@PVP0.5, and CDS-PMo12@PVP1 NPs. Figure S4 Optimized preparation of absorbent. (a) Adsorption time, (b) pH, (c) temperature and (d) ionic strength of B-R buffer on the adsorption efficiency of BSA, Hb, and Cyt-C. Protein solution: 100 μg/mL, 1.0 mL; CDS-PMo12@PVP0: 5.0 mg. Figure S5 Fluorescence emission spectra of (a-b) Cyt-C (d-e) Hb and (g-h) BSA decrease with the increasing amount of CDS-PMo12@PVP0 NPs at 298 K and 310 K. the maximum fluorescence intensity of (c) Cyt-C, (f) Hb and (i) BSA decrease in the presence of CDS-PMo12@PVP0 NPs (0-15 µM) at 298 K and 310 K, respectively. Figure S6 Fluorescence emission spectra of (a-b) Cyt-C (d-e) Hb and (g-h) BSA decrease with the increasing amount of CDS-PMo12@PVP1 NPs at 298 K and 310 K. the maximum fluorescence intensity of (c) Cyt-C, (f) Hb and (i) BSA decrease in the presence of CDS-PMo12@PVP1 NPs (0-15 µM) at 298 K and 310 K, respectively. Figure S7 Stern-Volmer plot derived from the fluorescence emission spectrum of the (a) Cyt-C, (b) Hb, and (c) BSA interaction with PMo12 NPs. The Modified Stern-Volmer plot of (d) Cyt-C, (e) Hb, and (f) BSA interaction with PMo12 NPs. The binding logarithmic graph of (g) Cyt-C, (h) Hb, and (i) BSA interaction with PMo12 NPs at 298 K and 310 K, respectively. Figure S8 The heatmap of 76 differential proteins of CDS-PMo12@PVP1 comparing to CDS-PMo12@PVP0, as identified by LC-MS/MS. Table S1 The top ten up-regulated and down-regulated proteins of 76 differential proteins.

Funding

Innovative Research Group Project of the National Natural Science Foundation of China Shanghai Municipal Science and Technology Project

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