11658_2022_386_MOESM1_ESM.docx (4.02 MB)
Additional file 1 of LncRNA SNHG16 promotes development of oesophageal squamous cell carcinoma by interacting with EIF4A3 and modulating RhoU mRNA stability
journal contributionposted on 2022-10-12, 03:22 authored by Lihua Ren, Xin Fang, Sachin Mulmi Shrestha, Qinghua Ji, Hui Ye, Yan Liang, Yang Liu, Yadong Feng, Jingwu Dong, Ruihua Shi
Additional file 1: Figure S1. EIF4A3 was upregulated in ESCC and promoted ESCC cell proliferation and migration. (A) Relative expression of EIF4A3 in human oesophageal cancer tissues (n = 162) compared with noncancerous tissues (n = 11) and a positive correlation with SNHG16 via the GEPIA database. (B) Western blot analysis of EIF4A3 after si-NC or si-EIF4A3 transfection in ESCC cells. Mock was the blank control group. GAPDH was used as an internal control. CCK-8 assays (C) and colony formation assays (D) were used to determine the proliferation ability of si-EIF4A3-transfected KYSE30 and KYSE410 cells. Transwell assays (E) and wound healing assays (F) were performed to investigate the migratory abilities of si-EIF4A3-transfected KYSE30 and KYSE410 cells. *P < 0.05, **P < 0.01, ***P < 0.001.