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Additional file 1 of Human endometrium-derived stem cell improves cardiac function after myocardial ischemic injury by enhancing angiogenesis and myocardial metabolism

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posted on 11.06.2021, 03:26 by Xuemei Fan, Sheng He, Huifang Song, Wenjuan Yin, Jie Zhang, Zexu Peng, Kun Yang, Xiaoyan Zhai, Lingxia Zhao, Hui Gong, Yi Ping, Xiangying Jiao, Sanyuan Zhang, Changping Yan, Hongliang Wang, Ren-Ke Li, Jun Xie
Additional file 1: Fig. S1. Results from RNA-seq and Protein array. a Heatmap from RNA-seq representing expression levels for differentially expressed genes (DEGs) between hBMSCs and hEMSCs. Red: upregulation; Blue: downregulation. Expression intensity was based on R software analysis of gene expression levels. b Twenty categories with significant enrichment were screened for functional enrichment analysis of gene ontology (GO). Yellow line: Number of genes. Bars: Significance level. c Heatmap showing up- and down-regulation for DEGs in hBMSCs and hEMSCs related to angiogenesis. d Venn diagram and protein array analysis between hBMSCs and hEMSCs for DEGs. Black circle in Venn diagram represents DEGs related to angiogenesis between hEMSCs and hBMSCs by RNA-seq, while gray circle represents DEGs identified through protein arrays. The intersection of the two circles represents overlapping DEGs between RNA-seq and protein array for angiogenesis, where five angiogenesis-related factors were found, of which PGF and ANG4 were up-regulated in hBMSCs, while FLT-1, TEK (also known as TIE2), and FGF9 were upregulated in hEMSCs. FGF9: Fibroblast growth factor 9; FLT-1: Fms-like tyrosine kinase 1; PGF: Placental growth factor; ANG4: Angiopoietin 4. Table S1. Formulation of Differentiation Media. Table S2. Real-time qPCR primer sets.

Funding

National Natural Science Foundation of China Natural Science Foundation for Young Scientists of Shanxi Province Key R&D program of Shanxi Province Fund for Shanxi Key Subjects Construction

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