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Additional file 1 of Development of a novel and rapid phenotype-based screening method to assess rice seedling growth

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posted on 16.10.2020, 03:28 by Lena Vlaminck, Chananchida Sang-Aram, Deborah Botterman, Christine Jewel C. Uy, Mary Kay Harper, Dirk Inzé, Godelieve Gheysen, Stephen Depuydt
Additional file 1: Figure S1. Workflow overview of the RIVA method. Seeds were sterilized and pregerminated by complete submergence in 2.0 mM CaSO4 in the dark. After 3 days of pregermination, selected seeds were transferred to test tubes filled with 1 mL 2.0 mM CaSO4 and a compound of interest. After 7 days, rice seedlings were harvested. Shoots and coleoptiles were transferred to plates and scanned. Yield-related parameters were automatically generated from these images via an in-house developed software, designated Plength.

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Universiteit Gent

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