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Additional file 1 of Developing Chenopodium ficifolium as a potential B genome diploid model system for genetic characterization and improvement of allotetraploid quinoa (Chenopodium quinoa)

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posted on 2021-10-26, 03:31 authored by Madhav Subedi, Erin Neff, Thomas M. Davis
Additional file 1: Figure S1. FTL amplicons in parents and hybrids. Gel electrophoresis result of identified hybrid plants along with the parents and controls from P x QC crosses. FTL gene primers were used for the PCR amplification and a 1Kb+ DNA ladder was used for the amplicon size identification in the first lane. True hybrids have both diagnostic bands, A1 and A2 (arrows). This image has been cropped. The uncropped image is provided in Figure S5-Additional file 2. Figure S2. FTL amplicons segregating in F2 population. Gel electrophoresis of FTL amplicons from P and QC parental plants, three putative hybrids (Ph), and the 25 F2 plants in the 1st experiment. The F2 plants are numbered from 1 to 25 in series from top and bottom gels and are arranged according to the flowering time (DAS). The positions of the diagnostic A1 and A2 bands are indicated by arrows to the left of the top gel. The lengths of the 1Kb+ DNA ladder bands used in both gels is represented in bp at the left of the bottom gel. This image has been cropped. The uncropped image is provided in Figure S6-Additional file 2. Figure S3. FTL amplicons segregating in F2 population. Gel electrophoresis results of three P, two QC, and 40 F2 individuals grown in the 2nd Experiment. The F2 plants are numbered from 1 to 40 in series and are arranged according to the flowering time. The plants were genotyped using the FTL locus marker. 1kb + DNA ladder was used for the amplicon size identification in first lane. This image has been cropped. The uncropped image is provided in Figure S7-Additional file 2. Figure S4. Cloning and gel extraction of FTL amplicons. Gel electrophoresis of cloned amplicons of FTL marker system. B, A2, C, and D (left), and the gel extracted amplicon A1 (right) of the FTL marker system. The P and QC lanes show the parental accessions amplicons as controls. The lengths of the 1Kb+ DNA ladder bands used in both gels is represented in bp at the left. This image has been cropped. The uncropped image is provided in Figure S8-Additional file 2.

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