Additional file 1: of Cooperative p16 and p21 action protects female astrocytes from transformation

Table S1. Real-time quantitative PCR primers and CRISPR guide RNA sequences. Table S2. Antibody details. Figure S1. Female and male CRISPR-IUE gliomas exhibit differences in histology. Figure S2. Sex differences in p21 and p27 expression in the CRISPR-IUE glioma model. Figure S3. Metaphase spreads from male and female GBM astrocytes grown under control (DMSO treated) conditions revealed aneuploidy. Figure S4. Western blot demonstration of p16, p21 and p27 deletion in Cas-9 expressing male and female GBM astrocytes expressing (+) or not expressing (−) the appropriate guide RNAs (gRNAs) as indicated. (PDF 3121 kb)