12915_2018_542_MOESM15_ESM.pdf (3.05 MB)
Download file

Additional file 15: of Structural complexity of the co-chaperone SGTA: a conserved C-terminal region is implicated in dimerization and substrate quality control

Download (3.05 MB)
journal contribution
posted on 11.07.2018, 05:00 by Santiago MartĂ­nez-Lumbreras, Ewelina Krysztofinska, Arjun Thapaliya, Alessandro Spilotros, Dijana Matak-Vinkovic, Enrico Salvadori, Peristera Roboti, Yvonne Nyathi, Janina Muench, Maxie Roessler, Dmitri Svergun, Stephen High, Rivka Isaacson
Figure S14. DEER measurements and distances determined for FL and NT-TPR SGTA constructs spin-labeled in the four SGTA mutants. (i) Primary frequency-domain DEER data. (ii) Background-corrected dipolar evolution data (black lines) and corresponding fits obtained through DeerAnalysis2016 [49] by Tikhonov regularization. (iii) Distance distributions obtained by Tikhonov regularization. Blue fits: FL SGTA; red fits: NT-TPR construct. (PDF 3125 kb)

Funding

Biotechnology and Biological Sciences Research Council

History