Absorption and MALDI-TOF spectra of αS pS129.

Absorbance spectra a) of acetylated αS and acetylated pS129 αS used to calculated concentrations of both proteins prior to MALDI-TOF (ε@280 nm = 5960 M^-1cm^-1). Both proteins were mixed in equimolar amounts and spotted for b) MADLI-TOF analysis. The relatively equal spectral peak heights of the MALDI-TOF spectra and the expected mass shift indicate that most, if not all, the αS is phosphorylated after the co-expression of NatB and PLK2 plasmids (see Materials and Methods for details).

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