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Download fileA Panel of Protein Kinase Chemosensors Distinguishes Different Types of Fatty Liver Disease
journal contribution
posted on 28.08.2019, 20:43 authored by Jon R. Beck, Fatima Cabral, Karuna Rasineni, Carol A. Casey, Edward N. Harris, Cliff I. StainsThe
worldwide incidence of fatty liver disease continues to rise,
which may account for concurrent increases in the frequencies of more
aggressive liver ailments. Given the existence of histologically identical
fatty liver disease subtypes, there is a critical need for the identification
of methods that can classify disease and potentially predict progression.
Herein, we show that a panel of protein kinase chemosensors can distinguish
fatty liver disease subtypes. These direct activity measurements highlight
distinct differences between histologically identical fatty liver
diseases arising from diets rich in fat versus alcohol and identify
a previously unreported decrease in p38α activity associated
with a high-fat diet. In addition, we have profiled kinase activities
in both benign (diet-induced) and progressive (STAM) disease models.
These experiments provide temporal insights into kinase activity during
disease development and progression. Altogether, this work provides
the basis for the future development of clinical diagnostics and potential
treatment strategies.
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Fatty Liver Diseaseliver ailmentsliver diseaseSTAMdisease modelsprotein kinase chemosensorsactivity measurementsfuture developmentkinase activityliver diseaseshigh-fat dietp 38α activityliver disease subtypesProtein Kinase Chemosensors Distinguishesdisease developmenttreatment strategieskinase activities