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A New Label-Free Continuous Fluorometric Assay for Trypsin and Inhibitor Screening with Tetraphenylethene Compounds

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journal contribution
posted on 2010-05-21, 00:00 authored by Wangxin Xue, Guanxin Zhang, Deqing Zhang, Daoben Zhu
A new label-free continuous assay with the ensemble of compound 1 and Arg6 for trypsin and inhibitor screening was successfully developed by taking advantage of the aggregation-induced emission feature of tetraphenylethene compounds. Both CLSM and DLS studies confirm the formation of the aggregation complex of compound 1 and Arg6 and disassembly after further addition of trypsin.