APPENDIX Detailed Technical protocol for Thorndyke et al.

APPENDIX Detailed Technical protocol to accompany Title: Development of Novel Technology for the Visualization and Quantitation of Angiogenesis and the Alveolar-Capillary Network in a Mouse Model of Fibrosis

Hannah Thorndyke^1*, Evan Lundberg^1*, Edwin Ortiz Gaxon², Maggie Dawson¹, Emma C. Mason¹, Julia M. Hollaway¹, Eszter K. Vladar³, David Coronado Escobar⁴ and Susan M. Majka^1,2,3,5*

Affiliations: ¹Department of Medicine, Division of Pulmonary, Critical Care & Sleep Medicine, National Jewish Health, Denver CO, 80206; ²Cell, Stem Cell and Development Graduate Program, University of Colorado Anschutz Medical Campus, Aurora, CO; ³Department of Medicine, University of Colorado Anschutz Medical Campus, Aurora, CO; ⁴Onimagin Technologies SCA, Cordoba, Spain; ⁵Gates Center for Regenerative Medicine and Stem Cell Biology, University of Colorado, Aurora, CO, 80045.