Pg 8-14 ALTERATION OF AMYLASE AND PHOSPHATASE.pdf (847.21 kB)

ALTERATION OF AMYLASE AND PHOSPHATASE IN Macrotyloma uniflorum UNDER INFLUENCE OF CADMIUM, MERCURY AND ZINC STRESSES

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journal contribution
posted on 18.01.2017, 19:14 by Khalid Naji, Varadahally R. Devaraj

Abstract

Physical weathering of parent rocks, mining, and other anthropogenic activities lead to impermissible levels of heavy metals in plant tissues. Effect of Cd2+, Hg2+, and Zn2+ on metabolic enzymes, Amylase (EC; 3.2.1.1), and AP (EC; 3.1.3.2), were studied to assess horse gram response to these heavy metal stress. Enzyme activity and TC were evaluated. Amylase decreased in response to increasing Cd2+ concentration, with great impact at 48-72h. It rose under 5 ppm Hg2+, while dropped under 10 ppm beyond 24h reaching ~25% of control, and recovered with time to reach 80-90% under 20 ppm. Under Zn2+ stress, amylase rose in concentration- and time-dependent manner. AP declined under metal stress in concentration, time-dependent manner, except at 100 ppm Zn2+. TC fluctuated in metal-specific manner with positive influence by Zn2+ (300-600 ppm) and Cd2+ (100-200 ppm) at 24 h. TC diminished under Cd2+ stress beyond 24 h, and over entire duration under Hg2+ stress. It can deduce that Hg2+ and Cd2+ exhibited toxic effects on horse gram while Zn2+ had positive effects. Amylase responded negatively to Hg2+, positively to Zn2+, and showed mixed response to Cd2+. AP levels decreased under metal stresses, except Zn2+.

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