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Download file3D Bioprinting of Carbohydrazide-Modified Gelatin into Microparticle-Suspended Oxidized Alginate for the Fabrication of Complex-Shaped Tissue Constructs
journal contribution
posted on 21.04.2020, 22:05 authored by Dong Nyoung Heo, Mecit Altan Alioglu, Yang Wu, Veli Ozbolat, Bugra Ayan, Madhuri Dey, Youngnam Kang, Ibrahim T. OzbolatExtrusion-based bioprinting
of hydrogels in a granular secondary
gel enables the fabrication of cell-laden three-dimensional (3D) constructs
in an anatomically accurate manner, which is challenging using conventional
extrusion-based bioprinting processes. In this study, carbohydrazide-modified
gelatin (Gel-CDH) was synthesized and deposited into a new multifunctional
support bath consisting of gelatin microparticles suspended in an
oxidized alginate (OAlg) solution. During extrusion, Gel-CDH and OAlg
were rapidly cross-linked because of the Schiff base formation between
aldehyde groups of OAlg and amino groups of Gel-CDH, which has not
been demonstrated in the domain of 3D bioprinting before. Rheological
results indicated that hydrogels with lower OAlg to Gel-CDH ratios
possessed superior mechanical rigidity. Different 3D geometrically
intricate constructs were successfully created upon the determination
of optimal bioprinting parameters. Human mesenchymal stem cells and
human umbilical vein endothelial cells were also bioprinted at physiologically
relevant cell densities. The presented study has offered a novel strategy
for bioprinting of natural polymer-based hydrogels into 3D complex-shaped
biomimetic constructs, which eliminated the need for cytotoxic supplements
as external cross-linkers or additional cross-linking processes, therefore
expanding the availability of bioinks.
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Keywords
OAlgRheological resultsDifferent 3 Dbioprinting parameterspolymer-based hydrogelsSchiff base formation3 D Bioprintingnovel strategy3 D bioprintingcross-linking processesHuman mesenchymalaldehyde groupsCarbohydrazide-Modified Gelatincarbohydrazide-modified gelatin3 D complex-shaped biomimeticComplex-Shaped Tissue Constructs Extrusion-based bioprintingMicroparticle-Suspended Oxidized Alginatecell densitiesGel-CDH ratiosmultifunctional support bathcytotoxic supplementsumbilical veinextrusion-based bioprinting processesgelatin microparticles