MegaPlex.pdf (683.89 kB)
MegaPlex PCR: a strategy for multiplex amplification
journal contribution
posted on 2008-04-07, 12:28 authored by Linda Strömqvist Meuzelaar, Owen Lancaster, J. Paul Pasche, Guido Kopal, Anthony J. Brookes‘MegaPlex PCR’ is a robust technology for highly multiplexed amplification of specific DNA sequences. It uses target-specific pairs of PCR primers that are physically separated by surface immobilization. Initial surface-based amplification cycles are then coupled to efficient solution-phase PCR using one common primer pair. We demonstrate this method by coamplifying and genotyping 75 unselected human single-nucleotide polymorphism (SNP) loci.