Recognition of the Nonpolar Base 4-Methylindole in DNA by the DNA Repair Adenine Glycosylase MutY
journal contributionposted on 14.04.2000 by Cindy Lou Chepanoske, Charles R. Langelier, Nikolas H. Chmiel, Sheila S. David
Any type of content formally published in an academic journal, usually following a peer-review process.
The DNA repair adenine glycosylase MutY efficiently recognizes 7-deaza-2‘-deoxyadenosine (Z) and its nonpolar isostere 4-methylindole β-deoxynucleoside (M) opposite 7,8-dihydro-8-oxo-2‘-deoxyguanosine (OG) and G in DNA. Both wild-type and truncated MutY exhibit a 10- to 20-fold higher affinity for a duplex containing OG:M than OG:Z. More efficient recognition of M over Z by MutY may be to due the lack of hydrogen bonding with the OG that facilitates nucleotide flipping during the substrate recognition process.