Mitoproteome Plasticity of Rat Brown Adipocytes in Response to Cold Acclimation
journal contributionposted on 05.01.2007 by Rachel Navet, Gregory Mathy, Pierre Douette, Rowan Laura Dobson, Pierre Leprince, Edwin De Pauw, Claudine Sluse-Goffart, Francis E. Sluse
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Cold acclimation induces an adaptative increase in respiration in brown adipose tissue (BAT). A comparative analysis by two-dimensional differential in-gel electrophoresis of mitochondrial protein patterns found in rat control and cold-acclimated BAT was performed. A total of 58 proteins exhibiting significant differences in their abundance was unambiguously identified. Proteins implicated in the major catabolic pathways were up-regulated as were ATP synthase and mitofilin. Moreover, these results support the fact that adipocytes can balance their ATP synthesis and their heat production linked to UCP1-sustained uncoupling. Keywords: Proteomics • 2 D-DIGE • Brown adipose tissue • Uncoupling protein 1 • Cold acclimation • Thermogenesis