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MOESM1 of Characterization of the aurantimycin biosynthetic gene cluster and enhancing its production by manipulating two pathway-specific activators in Streptomyces aurantiacus JA 4570

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posted on 21.09.2016, 05:00 by Houyuan Zhao, Liang Wang, Dan Wan, Jianzhao Qi, Rong Gong, Zixin Deng, Wenqing Chen
Additional file 1: Table S1. Strains, plasmids, and cosmid used in this study. Table S2. PCR primers used in this study. Table S3. ATM-A Yields of related strains after culturing for 5 days in shake-flask for regulatory genes inactivation. Table S4. ATM-A Yields of related strains after culturing for 5 days in shake-flask for regulatory genes overexpression. Figure S1. HPLC analysis of aurantimycin A. Figure S2. Alignment of ArtB and other regulators from Streptomyces. Figure S3. Construction of WL-02 mutant. Figure S4. Alignment of ArtX with other TetR regulators. Figure S5. Construction of WL-03 mutants. Figure S6. Identification of target gene overexpression strains. Figure S7. SDS-PAGE analysis of four purified A domains. Figure S8. Alignment of four KS domains in PKSs of the art cluster. Figure S9. Alignment of A domains activating piperazic acid.

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the National Science Foundation of China

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