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Integration of genetic and physical maps of the Primula vulgarisS locus and localization by chromosome in situ hybridization

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posted on 17.02.2016 by Pat Heslop-Harrison, J. Li, M. A. Webster, J. Wright, J. M. Cocker, M. C. Smith, Farah Badakshi, P. M. Gilmartin
Heteromorphic flower development in Primula is controlled by the S locus. The S locus genes, which control anther position, pistil length and pollen size in pin and thrum flowers, have not yet been characterized. We have integrated S-linked genes, marker sequences and mutant phenotypes to create a map of the P. vulgaris S locus region that will facilitate the identification of key S locus genes. We have generated, sequenced and annotated BAC sequences spanning the S locus, and identified its chromosomal location. We have employed a combination of classical genetics and three-point crosses with molecular genetic analysis of recombinants to generate the map. We have characterized this region by Illumina sequencing and bioinformatic analysis, together with chromosome in situ hybridization. We present an integrated genetic and physical map across the P. vulgaris S locus flanked by phenotypic and DNA sequence markers. BAC contigs encompass a 1.5-Mb genomic region with 1 Mb of sequence containing 82 S-linked genes anchored to overlapping BACs. The S locus is located close to the centromere of the largest metacentric chromosome pair. These data will facilitate the identification of the genes that orchestrate heterostyly in Primula and enable evolutionary analyses of the S locus.

History

Citation

New Phytologist, 2015, 208(1), pp. 137-148

Author affiliation

/Organisation/COLLEGE OF MEDICINE, BIOLOGICAL SCIENCES AND PSYCHOLOGY/MBSP Non-Medical Departments/Department of Genetics

Version

VoR (Version of Record)

Published in

New Phytologist

Publisher

Wiley for New Phytologist Trust

issn

0028-646X

eissn

1469-8137

Acceptance date

07/02/2015

Copyright date

2015

Available date

17/02/2016

Publisher version

http://onlinelibrary.wiley.com/doi/10.1111/nph.13373/abstract

Language

en

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