Deep-Red and Near-Infrared Xanthene Dyes for Rapid Live Cell Imaging
journal contributionposted on 03.08.2016 by Guangle Niu, Weimin Liu, Bingjiang Zhou, Hongyan Xiao, Hongyan Zhang, Jiasheng Wu, Jiechao Ge, Pengfei Wang
Any type of content formally published in an academic journal, usually following a peer-review process.
In this work, two xanthene dyes (H-hNR and TF-hNR) have been synthesized by a convenient and efficient method. These two dyes exhibited deep-red and near-infrared emissions, high fluorescence quantum yields, and good photostability. Their structure–optical properties were investigated by X-ray crystal structure analysis and density functional theory calculations. Live cell imaging data revealed that H-hNR and TF-hNR could rapidly stain both A549 and HeLa cells with low concentrations. The excellent photophysical and imaging properties render them as promising candidates for use in live cell imaging.