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Additional file 1: of Hidden in plain sight—highly abundant and diverse planktonic freshwater Chloroflexi

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posted on 02.10.2018, 05:00 by Maliheh Mehrshad, Michaela Salcher, Yusuke Okazaki, Shin-ichi Nakano, Karel Ĺ imek, Adrian-Stefan Andrei, Rohit Ghai
Figure S1. Maximum likelihood 16S rRNA tree reconstructed by adding the 16S rRNA sequences assembled from freshwater metagenomes to existing sequences of the SSURef_NR99_128 database in the phylum Chloroflexi. Figure S2. Percentage and distribution of Chloroflexi-related 16S rRNA reads (as % of total prokaryotic community) based on unassembled metagenomic datasets in brackish and marine datasets. Figure S3. Vertical profiles of water temperature, oxygen, phytoplankton and absolute CARD-FISH abundances of three lineages of Chloroflexi in Lake Zurich at five different sampling point in 2015. Figure S4. Vertical profiles of water temperature, oxygen, phytoplankton and absolute CARD-FISH abundances of three lineages of Chloroflexi in Rimov Reservoir at four different sampling points in 2015. Figure S5. Recruitment plot for ZSMay80m-G1 as a representative of the Chloroflexi CL500-11 cluster against different freshwater environments and the depth profile of brackish Caspian Sea. Figure S6. Recruitment plot for ZSMar2m-G89 as a representative of the Chloroflexi SL56 cluster against different freshwater environments. Figure S7. Recruitment plot for ZSMay80m-G79 as a representative of the Chloroflexi TK10 cluster against deep Caspian Sea dataset and different freshwater environments. Figure S8. Maximum likelihood tree of rhodopsin protein sequences from different bacterial and archaeal groups. Figure S9. Maximum likelihood tree of the pufM protein sequences from different bacterial groups. (PDF 3016 kb)

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